Composite

Part:BBa_K3431042:Design

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-10-17)


op21_B_ToeholdSwitch-Regulated Invertase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1421
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1192
    Illegal BamHI site found at 1322
    Illegal XhoI site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 524


Design Notes

We measure the thermodynamic characteristic of toehold switch and measure the glucose concentration with glucose meter to determine the efficacy of our composite device.


Source

This is a synthetic construct based on the work of Green et.al.

Green, A. A., Silver, P. A., Collins, J. J., & Yin, P. (2014). Toehold switches: de-novo-designed regulators of gene expression. Cell, 159(4), 925-939.

References